Protein Purification
STEMCELL Technologies RosetteSep™ Human T Cell Enrichment Cocktail, For processing: 200 mL whole blood processed
The RosetteSep™ Human T Cell Enrichment Cocktail is designed to isolate T cells from whole blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-T cells and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as RosetteSep™ DM-L (Catalog #15705) or Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The purified T cells are present as a highly enriched population at the interface between the plasma and the buoyant density medium.
STEMCELL Technologies RosetteSep™ Human NK Cell Enrichment Cocktail, For processing: 200 mL whole blood processed
The RosetteSep™ Human NK Cell Enrichment Cocktail is designed to isolate NK cells from whole blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-NK cells and red blood cells (RBCs). When centrifuged over a buoyant density medium such as RosetteSep™ DM-L (Catalog #15705) or Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The purified NK cells are present as a highly enriched population at the interface between the plasma and the buoyant density medium.
Bulldog Bio Inc GFPTRAP MAG AGAROSE 2.5ML
Reagent; Molecular Biology; GFP-Trap, Coupled to Magnetic Agarose Beads, for Immuno-Pulldown of GFP Fusions, 100 Reactions, 2.5ml
Zymo Research Corporation His-Spin Protein Miniprep™ Kit (50 Preps)
The simplified procedure is based on our innovative protein purification chemistry and custom designed fast spin columns. Up to 1 mg of His tagged protein can be purified in 5 minutes and eluted in as little as 100 ul of His-Elution Buffer. The purifiedprotein can be used directly for enzymatic assays, protein biochemical analyses, SDS-PAGE and other applications. The product has been optimized for maximal protein purity: a single protein band is visible by Coomassie blue staining on SDS-PAGE gel. The straightforward spin wash elute protocol dramatically simplifies protein purification: get results in minutes, not hours.
AdipoGen FABP1 human rec His
Protein. Human FABP1 aa 1-127 is fused at the N-terminus to a His-tag. Source E. coli. Endotoxin content <1EU/µg purified protein LAL test Lonza. Liquid. 0.2µm-filtered solution in 20mM Tris-HCl, pH 8. Purity >90% SDS-PAGE. FABP1 is a fatty acid binding protein in adipocytes. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. FABPs regulate the fatty acid uptake, transport, and metabolism. FABP1 is required for cholesterol synthesis and metabolism.
Invent Biotechnologies Inc Minute™ Total Protein Extraction Kit for Adipose Tissues/Cultured Adipocytes, 20 preps, spin-column-based, detergent-free extraction, high yield 2-3 mg/ml, 50-80 mg adipose tissue
Minute™ Total Protein Extraction Kit for Adipose Tissues/Cultured Adipocytes, 20 preps, spin-column-based, detergent-free extraction, high yield 2-3 mg/ml, 50-80 mg adipose tissue
AdipoGen Sortilin human rec His
Recombinant Protein. Human sortilin aa 78-755 is fused at the C-terminus to a His-tag. Source W2. Lyophilized. Contains PBS. Sortilin is a type I transmembrane multiligand receptor that is a member of the Vacuolar protein sorting 10 protein Vps10p domain receptor family. It is a 95 kDa protein, ubiquitously expressed, although most abundantly expressed in neurons, hepatocytes, adipocytes and white blood cells including macrophages. Sortilin is synthesized as a propeptide in the endoplasmic reticulum ER and processed to its mature form by furin-mediated cleavage in the trans-Golgi network. The primary function of sortilin is trafficking proteins from the Golgi to secretory vesicles and endolysosomal compartments. The majority of trafficking is from the Golgi to the endosomal compartment where sortilin deposits cargo targeted for catabolism in the lysosome and then is trafficked back to the Golgi via a retromer complex.
AdipoGen CD152 hrecHis
Recombinant Protein. Human CD152 aa 36-161 is fused at the C-terminus to a His-tag. Source W2. Lyophilized. Contains PBS. CD152 Cytotoxic T-lymphocyte-associated protein 4 CTLA-4 is an inhibitory receptor belonging to the CD28 immunoglobulin subfamily, expressed primarily by T-cells. Its ligands, CD80 and CD86, are typically found on the surface of antigen-presenting cells and can either bind CD28 or CTLA-4, resulting in a costimulatory or a co-inhibitory response, respectively. Because of its dampening effect, CTLA-4 is a crucial regulator of T-cell homeostasis and self-tolerance. While CD28 promotes T-cell activation and proliferation, CTLA-4 is reported to dampen T-cell responses through a variety of mechanisms. Prior to activation, conventional T-cells Tconv express low levels of CTLA-4, predominantly in intracellular compartments. Upon activation, CTLA-4 expression is upregulated and becomes increasingly detectable on the cell surface.
Sigma Aldrich Fine Chemicals Biosciences ANTI-FLAG M2 Affinity Gel purified immunoglobulin, buffered aqueous glycerol solution, 56-81-5
ANTI-FLAG M2 Affinity Gel purified immunoglobulin, buffered aqueous glycerol solution
Zymo Research Corporation Zymo-Spin IICR Columns, 5x50 pack
The Zymo-Spin IICR features durable polypropylene construction with an attached cap and contains a unique silica-based matrix that allows purification of up to 25 µg of DNA or 50 µg of RNA, in ≥ 25 µl eluate. Capacity is 900 µl.
Zymo Research Corporation ZymoBIOMICS™ MagBinding Beads (6 mL)
ZymoBIOMICS MagBinding Beads. For use in ZymoBIOMICS magenetic bead-based purification kits. Sold individually or as part of the ZymoBIOMICS product line.